Recipes > Annealing Buffer 2X, 1 mL pH 8.3

Ingredients

Name Stock MW or Conc. Final Conc. Weight or Volume
KCl 2 M 500 mM 250 ul
Tris (1 M, pH 8.3) 1 M 50 mM 50 ul
ddH2O 700 ul

Preparation

Mix the ingredients above for a 1 ml stock solution.