Recipes > Denhardt’s solution 100X, 50ml

Ingredients

Name Stock MW or Conc. Final Conc. Weight or Volume
Bovine serum albumin (fraction V) 2% 1 g
Ficoll 400 2% 1g
Polyvinylpyrrolidone (PVP) 2% 1g
ddH2O up to 50ml

Preparation

1 Dissolve the BSA, Ficoll and PVP in the ddH2O.
2 Filter to sterilize and remove particulate matter.
3 Divide into aliquots, and store at −20°C.