Recipes > HEPES buffer, 1L pH 7.4

Ingredients

Name Stock MW or Conc. Final Conc. Weight or Volume
NaCl 58.44 g/mol 115mM 6.72 g
CaCl2 110.98 g/mol 1.2 mM 133 mg
MgCl2 95.211 g/mol 1.2 mM 114 mg
K2HPO4 174.2 g/mol 2.4 mM 418 mg
HEPES 238.3012 g/mol 20mM 4.77 g
ddH2O (up to) 1L

Preparation

1 Combine all the reagents above.
2 Adjust the pH to 7.4 with HCl or NaOH.