Recipes > Laemmli buffer 6X , 10ml

Ingredients

Name Stock MW or Conc. Final Conc. Weight or Volume
SDS 1.2g
Bromophenol blue 6mg
Glycerol 4.7ml
Tris pH6.8 0.5M 1.2ml
ddH2O 2.1ml
DTT 0.93g

Preparation

Laemmli is a sample buffer to use in western blot. 5µl of protein sample + 1µl of 6X Laemmli buffer is boiled or heated for 5-10 minutes.

1 Warm all the ingredients except the DTT and shake it till everything is dissolved. (Takes time).
2 Add the DTT.
3 Make sure everything is dissolved; then aliquot and freeze.