Recipes > LB Agar (for plates), 1L pH 7.5

Ingredients

Name Stock MW or Conc. Final Conc. Weight or Volume
ddH2O 800ml
Bacto-tryptone 10g
NaCl 10g
Yeast extract 5g
Agar 15g

Preparation

1 Add the Bacto-tryptone, NaCl to the ddH2O.
2 Adjust pH to 7.5 with NaOH.
3 Add the 15g of agar.
4 Melt agar into solution in the microwave.
5 Adjust volume to 1L with ddH2O.
6 Sterilize by autoclaving.
7 Cool to 55C.
8 Add antibiotic if required.
9 Pour into sterile petri dishes (approx. 20 ml/plate).